Journal: JCI Insight

Article Title: Lipid abnormalities in atopic skin are driven by type 2 cytokines

doi: 10.1172/jci.insight.98006

Figure Lengend Snippet: Keratinocytes were differentiated in the absence or presence of IL-4/IL-13. Keratinocytes were transfected with control siRNA, STAT6 siRNA, ELOVL3 siRNA, ELOVL6 siRNA, or ELOVL3/ELOVL6 siRNA and underwent Ca2+ differentiation for the period of time indicated. The expressions of STAT6 mRNA (A and D), ELOVL3 mRNA (B and E), and ELOVL6 (C and F) were analyzed. (G) ELOVL3 immunostaining in keratinocytes. ELOVL3 staining, yellow; DAPI, cell nuclei staining, blue. Magnification, 250×. *P < 0.05, **P < 0.01, 2-tailed Student’s t test (n = 3).

Article Snippet: After blocking with SuperBlock (SkyTec), cells were incubated with anti-ELOVL3 antibody (catalog ab110872, Abcam) overnight at 4°C and washed 3 times with PBS/0.05% Triton X-100, followed by incubation with FITC conjugated donkey an–-rabbit F(ab’)2 antibody (catalog 711-096-152, Jackson ImmunoResearch Laboratories) and DAPI cell nuclei stain (MilliporeSigma) for 1 hour at room temperature.

Techniques: Transfection, Control, Immunostaining, Staining

Journal: eLife

Article Title: Endothelial cell signature in muscle stem cells validated by VEGFA-FLT1-AKT1 axis promoting survival of muscle stem cell

doi: 10.7554/eLife.73592

Figure Lengend Snippet:

Article Snippet: DAPI (10236276001, MilliporeSigma) was used for counterstaining of nuclei.

Techniques: Plasmid Preparation, RNAscope, Sequencing, Imaging, Staining, Crystal Violet Assay, Viability Assay, cDNA Synthesis, Isolation, DNA Extraction, Western Blot, Extraction, Protein Extraction, Modification, Recombinant, Transfection, Infection, Immunostaining, Software, Microscopy, Cell Culture